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Purification and Properties of Polyphenol Oxidase of Dried Volvariella bombycina

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dc.contributor.author Sarsenova, Assemgul
dc.contributor.author Demir, Dudu
dc.contributor.author Ça ˘glayan, Kardelen
dc.contributor.author Abiyev, Sardarbek
dc.contributor.author Darbayeva, Talshen
dc.contributor.author Eken, Cafer
dc.date.accessioned 2024-09-10T06:40:46Z
dc.date.available 2024-09-10T06:40:46Z
dc.date.issued 2023
dc.identifier.citation Sarsenova, A.; Demir, D.; Ça ˘glayan, K.; Abiyev, S.; Darbayeva, T.; Eken, C. Purification and Properties of Polyphenol Oxidase of Dried Volvariella bombycina. Biology 2023, 12, 53. https://doi.org/ 10.3390/biology12010053 ru
dc.identifier.issn 2079-7737
dc.identifier.other doi.org/10.3390/biology12010053
dc.identifier.uri http://rep.enu.kz/handle/enu/16155
dc.description.abstract Polyphenol oxidase (PPO) was purified and characterized from a dried wild edible and medicinal mushroom (V. bombycina). Using Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity chromatography, PPO was purified from the dried V. bombycina. The purification was completed with a 33.85-fold purification. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme migrated as a single band. The molecular weight of the purified enzyme was estimated by SDS-PAGE to be about 25 kDa. Catechol, 4-methyl catechol, and pyrogallol were used as substrates to determine the enzyme activity and its kinetic parameters (Km and Vmax). At the optimum pH and temperature, dried V. bombycina PPO’s Km and Vmax values for catechol, 4-methyl catechol, and pyrogallol were found to be 1.67 mM–833.33 U/mL, 3.17 mM–158.73 U/mL, and 2.67 mM–3333.33 U/mL, respectively. Also investigated were the effects of pH and temperature on the enzymatic properties of PPO in dried V. bombycina. The optimum pH and temperature values for dried V. bombycina PPO obtained by using catechol, 4-methyl catechol, and pyrogallol as substrates were 6.5, 15 ◦C; 9.0, 20 ◦C; and 8.0, 15◦C, respectively. This is the first study on the purification and characterization of PPO from dried V. bombycina. ru
dc.language.iso en ru
dc.publisher Biology ru
dc.relation.ispartofseries Volume 12;Issue 1
dc.subject Volvariella bombycina ru
dc.subject polyphenol oxidase ru
dc.subject affinity chromatography ru
dc.subject purification ru
dc.subject characterization ru
dc.title Purification and Properties of Polyphenol Oxidase of Dried Volvariella bombycina ru
dc.type Article ru


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