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Method for assessing the content of molybdenum enzymes in the internal organs of fish

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dc.contributor.author Satkanov, Mereke
dc.contributor.author Tazhibay, Diana
dc.contributor.author Zhumabekova, Bibigul
dc.contributor.author Assylbekova, Gulmira
dc.contributor.author Abdukarimov, Nurzhan
dc.contributor.author Nurbekova, Zhadyrassyn
dc.contributor.author Kulatayeva, Maral
dc.contributor.author Aubakirova, Karlygash
dc.contributor.author Alikulov, Zerekbai
dc.date.accessioned 2024-09-11T04:33:04Z
dc.date.available 2024-09-11T04:33:04Z
dc.date.issued 2024-01
dc.identifier.issn 2215-0161
dc.identifier.other doi.org/10.1016/j.mex.2024.102576
dc.identifier.uri http://rep.enu.kz/handle/enu/16181
dc.description.abstract Molybdenum enzymes (Mo-enzymes) contain a molybdenum cofactor (MoCo) in the active site. These enzymes are potentially interesting for studying the survival mechanism of fish under hypoxic water conditions. This is because Mo-enzymes can synthesize nitric oxide from nitrates and nitrites, which are present in high concentrations under hypoxic water conditions. However, there is currently no method for assessing the Mo-enzymes content in the fish internal organs. Methods capable of determining Mo-enzymes content in the fish are of major importance. For this purpose, a method for quantitative determination of MoCo from plant tissues was modified. We demonstrated the Mo-enzyme content assessment by isolated MoCo from the fish’s internal organs and the Neurospora crassa nit-1 extract containing inactive NADPH nitrate reductase. The Mo enzyme content was calculated using a calibration curve in nM of nitrites as a product of restored NADPH reductase activity after complementation with MoCo. Here we present a robust laboratory method which can be used to assess the content of Mo-enzymes in the internal organs of fish. ru
dc.language.iso en ru
dc.publisher MethodsX ru
dc.relation.ispartofseries 12;102576
dc.subject Method for fish biochemistry ru
dc.subject Molybdenum cofactor ru
dc.subject Neurospora crassa nit-1 ru
dc.subject Fish molybdenum enzymes ru
dc.subject Fish liver enzymes ru
dc.subject Fish intestines enzymes ru
dc.title Method for assessing the content of molybdenum enzymes in the internal organs of fish ru
dc.type Article ru


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