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miR‑19 in blood plasma reflects lung cancer occurrence but is not specifically associated with radon exposure

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dc.contributor.author BULGAKOVA, OLGA
dc.contributor.author ZHABAYEVA, DINARA
dc.contributor.author KUSSAINOVA, ASSIYA
dc.contributor.author PULLIERO, ALESSANDRA
dc.contributor.author IZZOTTI, ALBERTO
dc.contributor.author BERSIMBAEV, RAKHMETKAZHI
dc.date.accessioned 2024-09-12T05:19:12Z
dc.date.available 2024-09-12T05:19:12Z
dc.date.issued 2018
dc.identifier.issn 1792-1074
dc.identifier.other doi.org/10.3892/ol.2018.8392
dc.identifier.uri http://rep.enu.kz/handle/enu/16230
dc.description.abstract Radon is one of the most powerful carcinogens, particularly in terms of lung cancer onset and development. miRNAs may be considered not only as markers of the ongoing tumorigenesis but also as a hallmark of exposure to radiation, including radon and its progeny. Therefore, the purpose of the present study was to estimate the value of plasma miR-19b-3p level as the prospective marker of the response to radon exposure in lung cancer pathogenesis. A total of 136 subjects were examined, including 49 radon-exposed patients with lung cancer, 37 patients with lung cancer without radon exposure and 50 age/sex matched healthy controls. Total RNA from blood samples was extracted and used to detect miR-19b-3p expression via reverse transcription quantitative-polymerase chain reaction. The 2-ΔΔCq method was used to quantify the amount of relative miRNA. The plasma level of p53 protein was determined using a Human p53 ELISA kit. Plasma miR-19b-3p level was significantly higher in the patients with lung cancer groups, compared with the healthy control group (P<0.0001). No other statistically significant differences were determined in the expression level of plasma miR-19b-3p between patients diagnosed with lung cancer exposed to radon and not exposed to radon. The expression level of free circulating miR-19b-3p was higher in the group of non-smoking patients with lung cancer, compared with smokers with lung cancer. The miR-19b-3p was 1.4-fold higher in non-smokers than in smokers (P<0.05). No association between plasma levels of p53 protein and miR-19b-3p freely circulating in patients with lung cancer was observed. No other statistically significant differences were determined in the plasma p53 protein level between patients diagnosed with lung cancer exposed and not exposed to radon. These results indicated that detection of miR-19b-3p levels in plasma potentially could be exploited as a noninvasive method for the lung cancer diagnostics. However, this miRNA is not suitable as the precise marker for radon impact. ru
dc.language.iso en ru
dc.publisher ONCOLOGY LETTERS ru
dc.relation.ispartofseries 15;8816-8824
dc.subject miRNA ru
dc.subject lung cancer ru
dc.subject biomarker ru
dc.subject radon ru
dc.subject p53 ru
dc.title miR‑19 in blood plasma reflects lung cancer occurrence but is not specifically associated with radon exposure ru
dc.type Article ru


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