DSpace Repository

Prevalence of Beijing Central Asian/Russian Cluster 94-32 among Multidrug-Resistant M. tuberculosis in Kazakhstan

Show simple item record

dc.contributor.author Akhmetova, Ainur
dc.contributor.author Bismilda, Venera
dc.contributor.author Chingissova, Lyailya
dc.contributor.author Filipenko, Maxim
dc.contributor.author Akilzhanova, Ainur
dc.contributor.author Kozhamkulov, Ulan
dc.date.accessioned 2024-09-12T06:49:08Z
dc.date.available 2024-09-12T06:49:08Z
dc.date.issued 2024
dc.identifier.citation Akhmetova, A.; Bismilda, V.; Chingissova, L.; Filipenko, M.; Akilzhanova, A.; Kozhamkulov, U. Prevalence of Beijing Central Asian/Russian Cluster 94-32 among Multidrug-Resistant M. tuberculosis in Kazakhstan. Antibiotics 2024, 13, 9. https://doi.org/10.3390/ antibiotics13010009 ru
dc.identifier.issn 2079-6382
dc.identifier.other doi.org/10.3390/antibiotics13010009
dc.identifier.uri http://rep.enu.kz/handle/enu/16239
dc.description.abstract The Beijing genotype is the most distributed M. tuberculosis family in Kazakhstan. In this study, we identified dominant Beijing clusters in Kazakhstan and assessed their drug susceptibility profiles and association with the most widely spread mutation Ser531Leu of the rpoB gene and the mutation Ser315Thr of the katG gene associated with resistance to rifampicin and isoniazid, respectively. M. tuberculosis isolates (n = 540) from new TB cases were included in the study. MIRUVNTR genotyping was performed for 540 clinical isolates to determine M. tuberculosis families using 24 loci. RD analysis was additionally performed for the Beijing isolates. The identification of mutations in the drug-resistance genes of M. tuberculosis was performed with allele-specific realtime PCR and Sanger sequencing. The Beijing genotype was identified in 60% (324/540) of the clinical isolates. Central Asian/Russian cluster 94-32 was the most distributed cluster among the Beijing isolates (50.3%; 163/324). Three other dominant Beijing clusters were identified as 94-33 (3.4%; 11/324), 100-32 (3.1%; 10/324) and 99-32 (3.1%; 10/324). The Beijing genotype was associated with drug-resistant TB (p < 0.0001), including multidrug-resistant TB (p < 0.0001), in our study. An association of the mutation Ser531Leu of the rpoB gene with the Beijing genotype was found (p < 0.0001; OR = 16.0000; 95%CI: 4.9161–52.0740). Among the Beijing isolates, cluster 94-32 showed an association with MDR-TB (p = 0.021). This is why the evaluation of the Beijing genotype and its clusters is needed to control MDR-TB in Kazakhstan. ru
dc.language.iso en ru
dc.publisher Antibiotics ru
dc.relation.ispartofseries Volume 13;Issue 1
dc.subject pulmonary tuberculosis ru
dc.subject Beijing genotype ru
dc.subject Central Asian/Russian type 94-32 ru
dc.subject MIRU-VNTR ru
dc.subject multidrug-resistance ru
dc.title Prevalence of Beijing Central Asian/Russian Cluster 94-32 among Multidrug-Resistant M. tuberculosis in Kazakhstan ru
dc.type Article ru


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Browse

My Account