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Comparative Characterization and Identification of Poly-3-hydroxybutyrate Producing Bacteria with Subsequent Optimization of Polymer Yield

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dc.contributor.author Rysbek, Aidana
dc.contributor.author Ramankulov, Yerlan
dc.contributor.author Kurmanbayev, Askar
dc.contributor.author Richert, Agnieszka
dc.contributor.author Abeldenov, Sailau
dc.date.accessioned 2024-09-19T09:56:53Z
dc.date.available 2024-09-19T09:56:53Z
dc.date.issued 2022
dc.identifier.citation Rysbek, A.; Ramankulov, Y.; Kurmanbayev, A.; Richert, A.; Abeldenov, S. Comparative Characterization and Identification of Poly-3-hydroxybutyrate Producing Bacteria with Subsequent Optimization of Polymer Yield. Polymers 2022, 14, 335. https:// doi.org/10.3390/polym14020335 ru
dc.identifier.issn 2073-4360
dc.identifier.other doi.org/10.3390/polym14020335
dc.identifier.uri http://rep.enu.kz/handle/enu/16670
dc.description.abstract In this work, the strains Bacillus megaterium RAZ 3, Azotobacter chrocococcum Az 3, Bacillus araybhattay RA 5 were used as an effective producer of poly-3-hydroxybutyrate P(3HB). The purpose of the study was to isolate and obtain an effective producer of P(3HB) isolated from regional chestnut soils of northern Kazakhstan. This study demonstrates the possibility of combining the protective system of cells to physical stress as a way to optimize the synthesis of PHA by strains. Molecular identification of strains and amplification of the phbC gene, transmission electron microscope (TEM), extracted and dried PHB were subjected to Fourier infrared transmission spectroscopy (FTIR). The melting point of the isolated P(3HB) was determined. The optimal concentration of bean broth for the synthesis of P(3HB) for the modified type of Bacillus megaterium RAZ 3 was 20 g/L, at which the dry weight of cells was 25.7 g/L−1 and P(3HB) yield of 13.83 g/L−1 , while the percentage yield of P(3HB) was 53.75%. The FTIR spectra of the extracted polymer showed noticeable peaks at long wavelengths. Based on a proof of concept, this study demonstrates encouraging results. ru
dc.language.iso en ru
dc.publisher Polymers ru
dc.relation.ispartofseries 14;335
dc.subject P(3HB) ru
dc.subject bean broth ru
dc.subject ultraviolet irradiation ru
dc.subject molecular characterization ru
dc.subject biopolymers ru
dc.subject TEM ru
dc.title Comparative Characterization and Identification of Poly-3-hydroxybutyrate Producing Bacteria with Subsequent Optimization of Polymer Yield ru
dc.type Article ru


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