Abstract:
Potato (Solanum tuberosum L.) is the third most economically important crop in the
world and has a high nutritional value. In this study, the in vitro culture response of four
widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar,
and Aksor, was investigated using stem and leaf explants. Published protocols were
evaluated and optimized to develop a more efficient protocol for the regeneration of
plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate
potato genome modification. The explants were cultured on solid Murashige and Skoog
medium supplemented with different concentrations and combinations of zeatin, 6-
benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA)
and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem
internodal explants. A significant effect of the explant source on direct regeneration was
confirmed with statistical analysis. The number of shoots obtained from the internode
was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRMVIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct
shoot regeneration and multiple shoot formation from all cultivars. To conclude, we
outline a protocol for direct plant regeneration from four potato cultivars. Our findings
suggest commercial cultivars Astanalyk and Aksor are good candidates for developing
the genome-edited plants through direct shoot regeneration.
