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High frequency direct shoot regeneration from Kazakh commercial potato cultivars

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dc.contributor.author Abeuova, Laura S.
dc.contributor.author Kali, Balnur R.
dc.contributor.author Rakhimzhanova, Aizhan O.
dc.contributor.author Bekkuzhina, Sara S.
dc.contributor.author Manabayeva, Shuga A.
dc.date.accessioned 2024-09-09T12:08:06Z
dc.date.available 2024-09-09T12:08:06Z
dc.date.issued 2020-07
dc.identifier.citation Abeuova LS, Kali BR, Rakhimzhanova AO, Bekkuzhina SS, Manabayeva SA. 2020. High frequency direct shoot regeneration from Kazakh commercial potato cultivars. PeerJ 8:e9447 https://doi.org/10.7717/peerj.9447 ru
dc.identifier.issn 2167-8359
dc.identifier.other DOI 10.7717/peerj.9447
dc.identifier.uri http://rep.enu.kz/handle/enu/16128
dc.description.abstract Potato (Solanum tuberosum L.) is the third most economically important crop in the world and has a high nutritional value. In this study, the in vitro culture response of four widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar, and Aksor, was investigated using stem and leaf explants. Published protocols were evaluated and optimized to develop a more efficient protocol for the regeneration of plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate potato genome modification. The explants were cultured on solid Murashige and Skoog medium supplemented with different concentrations and combinations of zeatin, 6- benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem internodal explants. A significant effect of the explant source on direct regeneration was confirmed with statistical analysis. The number of shoots obtained from the internode was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRMVIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct shoot regeneration and multiple shoot formation from all cultivars. To conclude, we outline a protocol for direct plant regeneration from four potato cultivars. Our findings suggest commercial cultivars Astanalyk and Aksor are good candidates for developing the genome-edited plants through direct shoot regeneration. ru
dc.language.iso en ru
dc.publisher PeerJ ru
dc.subject Solanum tuberosum L. ru
dc.subject Tissue culture ru
dc.subject Direct regeneration ru
dc.subject Plant growth regulator ru
dc.subject Microtuber ru
dc.title High frequency direct shoot regeneration from Kazakh commercial potato cultivars ru
dc.type Article ru


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