Abstract:
The biological variety of aquatic ecosystems is significantly impacted by the chemical and
microbiological composition of water bodies, and there is strong reciprocal feedback between these
two factors, especially for reservoirs, which can and do have a significant impact on neighboring
ecosystems. Today there is an acute need to identify the most effective and economically feasible
methods for cleaning and restoring water bodies. Therefore, the aim of the study was to find strains
of microorganisms which are capable of biodegrading such problematic pollutants as insoluble
phosphates and excess nitrogenous compounds and at the same time, are capable of suppressing the
bacterial composition in reservoir waters. In the course of the research, a number of the most active
strains of microorganisms were isolated from lake water samples. Five isolates were obtained from
salt water and the isolates were then identified using morphological, and biochemical techniques, as
well as the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF
MS). In addition to the characterization and identification of the isolates, the species-specific levels
of phosphate solubilizing, and nitrifying activities were also established. Thus, all isolated strains
were studied and characterized, and their influence on the content of minerals such as phosphorus
and nitrogen, which are important for living organisms in water, was studied. In summary, the
Pseudomonas Extrem-Orientalis isolate was observed to be highly effective in solubilizing phosphates,
nitrifying, and had the greatest antagonistic capacity among the investigated isolates. The information
gleaned from the study’s findings helps raise more awareness in the field of microbiology and water
treatment in general. The findings offer promise for the development of biopreparations with
bioremediation capabilities for cleaning polluted water bodies of pollutants from various sources.
