Abstract:
In this work, the strains Bacillus megaterium RAZ 3, Azotobacter chrocococcum Az 3, Bacillus
araybhattay RA 5 were used as an effective producer of poly-3-hydroxybutyrate P(3HB). The purpose
of the study was to isolate and obtain an effective producer of P(3HB) isolated from regional chestnut
soils of northern Kazakhstan. This study demonstrates the possibility of combining the protective
system of cells to physical stress as a way to optimize the synthesis of PHA by strains. Molecular
identification of strains and amplification of the phbC gene, transmission electron microscope (TEM),
extracted and dried PHB were subjected to Fourier infrared transmission spectroscopy (FTIR). The
melting point of the isolated P(3HB) was determined. The optimal concentration of bean broth for the
synthesis of P(3HB) for the modified type of Bacillus megaterium RAZ 3 was 20 g/L, at which the dry
weight of cells was 25.7 g/L−1 and P(3HB) yield of 13.83 g/L−1
, while the percentage yield of P(3HB)
was 53.75%. The FTIR spectra of the extracted polymer showed noticeable peaks at long wavelengths.
Based on a proof of concept, this study demonstrates encouraging results.
